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normal human colon epithelial cell model  (ATCC)
97
ATCC normal human colon epithelial cell model
Cytotoxic response and intracellular ROS levels induced by the multimodal hydrogel nanoplatform in CRC and normal colon cell models. (A) Dose–response curve of free 5-FU in HCT-116 cells after 48 h, determined by MTT assay and used to define the experimental IC₅₀. Cell viability was normalized to the Ctrl. For visualization on a logarithmic x-axis, the Ctrl condition is plotted at a nominal concentration of 0.1 µg mL⁻¹. (B–D) MTT-based cell viability after 48 h treatment in (B) HCT-116, (C) SW480, and (D) normal human colon <t>epithelial</t> NCM460 cells. Treatment groups include Ctrl, free 5-FU (5FU), non-targeted nanoparticles (NP), HA-targeted nanoparticles (tNP), blank hydrogel (Gel), hydrogel-loaded targeted nanoparticles (Gel-tNP), and Gel-tNP combined with near-infrared irradiation (NIR, 808 nm), X-ray irradiation (XR, 2 Gy), or both. All treatments were administered at an equivalent 5-FU concentration corresponding to the IC₅₀ determined in panel (A). (E) Intracellular ROS levels in HCT-116 cells measured after 48 h using CellROX™ Deep Red and expressed as fold change relative to Ctrl. Data are mean ± SD ( n = 3). Statistical significance was assessed by one-way ANOVA with multiple-comparisons post-hoc testing. Asterisks indicate comparisons vs. Ctrl (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Brackets indicate the specific intergroup comparisons shown (e.g., dual-modal vs. tri-modal where indicated). “ns” denotes not significant
Normal Human Colon Epithelial Cell Model, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/airway+model/pmc12977890-142-19-30?v=ATCC
Average 97 stars, based on 1 article reviews
normal human colon epithelial cell model - by Bioz Stars, 2026-07
97/100 stars
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human airway epithelial tissue model  (Epithelix)
86
Epithelix human airway epithelial tissue model
Cytotoxic response and intracellular ROS levels induced by the multimodal hydrogel nanoplatform in CRC and normal colon cell models. (A) Dose–response curve of free 5-FU in HCT-116 cells after 48 h, determined by MTT assay and used to define the experimental IC₅₀. Cell viability was normalized to the Ctrl. For visualization on a logarithmic x-axis, the Ctrl condition is plotted at a nominal concentration of 0.1 µg mL⁻¹. (B–D) MTT-based cell viability after 48 h treatment in (B) HCT-116, (C) SW480, and (D) normal human colon <t>epithelial</t> NCM460 cells. Treatment groups include Ctrl, free 5-FU (5FU), non-targeted nanoparticles (NP), HA-targeted nanoparticles (tNP), blank hydrogel (Gel), hydrogel-loaded targeted nanoparticles (Gel-tNP), and Gel-tNP combined with near-infrared irradiation (NIR, 808 nm), X-ray irradiation (XR, 2 Gy), or both. All treatments were administered at an equivalent 5-FU concentration corresponding to the IC₅₀ determined in panel (A). (E) Intracellular ROS levels in HCT-116 cells measured after 48 h using CellROX™ Deep Red and expressed as fold change relative to Ctrl. Data are mean ± SD ( n = 3). Statistical significance was assessed by one-way ANOVA with multiple-comparisons post-hoc testing. Asterisks indicate comparisons vs. Ctrl (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Brackets indicate the specific intergroup comparisons shown (e.g., dual-modal vs. tri-modal where indicated). “ns” denotes not significant
Human Airway Epithelial Tissue Model, supplied by Epithelix, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/airway+model/pm42273851-333-14-20?v=Epithelix
Average 86 stars, based on 1 article reviews
human airway epithelial tissue model - by Bioz Stars, 2026-07
86/100 stars
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reconstructed human upper airway model mucilairtm advanced toxicological  (Epithelix)
86
Epithelix reconstructed human upper airway model mucilairtm advanced toxicological
Cytotoxic response and intracellular ROS levels induced by the multimodal hydrogel nanoplatform in CRC and normal colon cell models. (A) Dose–response curve of free 5-FU in HCT-116 cells after 48 h, determined by MTT assay and used to define the experimental IC₅₀. Cell viability was normalized to the Ctrl. For visualization on a logarithmic x-axis, the Ctrl condition is plotted at a nominal concentration of 0.1 µg mL⁻¹. (B–D) MTT-based cell viability after 48 h treatment in (B) HCT-116, (C) SW480, and (D) normal human colon <t>epithelial</t> NCM460 cells. Treatment groups include Ctrl, free 5-FU (5FU), non-targeted nanoparticles (NP), HA-targeted nanoparticles (tNP), blank hydrogel (Gel), hydrogel-loaded targeted nanoparticles (Gel-tNP), and Gel-tNP combined with near-infrared irradiation (NIR, 808 nm), X-ray irradiation (XR, 2 Gy), or both. All treatments were administered at an equivalent 5-FU concentration corresponding to the IC₅₀ determined in panel (A). (E) Intracellular ROS levels in HCT-116 cells measured after 48 h using CellROX™ Deep Red and expressed as fold change relative to Ctrl. Data are mean ± SD ( n = 3). Statistical significance was assessed by one-way ANOVA with multiple-comparisons post-hoc testing. Asterisks indicate comparisons vs. Ctrl (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Brackets indicate the specific intergroup comparisons shown (e.g., dual-modal vs. tri-modal where indicated). “ns” denotes not significant
Reconstructed Human Upper Airway Model Mucilairtm Advanced Toxicological, supplied by Epithelix, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/airway+model/10__3390_slash_polym18030374-222-2-17?v=Epithelix
Average 86 stars, based on 1 article reviews
reconstructed human upper airway model mucilairtm advanced toxicological - by Bioz Stars, 2026-07
86/100 stars
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airway infection model  (Charles River Laboratories)
86
Charles River Laboratories airway infection model
Cytotoxic response and intracellular ROS levels induced by the multimodal hydrogel nanoplatform in CRC and normal colon cell models. (A) Dose–response curve of free 5-FU in HCT-116 cells after 48 h, determined by MTT assay and used to define the experimental IC₅₀. Cell viability was normalized to the Ctrl. For visualization on a logarithmic x-axis, the Ctrl condition is plotted at a nominal concentration of 0.1 µg mL⁻¹. (B–D) MTT-based cell viability after 48 h treatment in (B) HCT-116, (C) SW480, and (D) normal human colon <t>epithelial</t> NCM460 cells. Treatment groups include Ctrl, free 5-FU (5FU), non-targeted nanoparticles (NP), HA-targeted nanoparticles (tNP), blank hydrogel (Gel), hydrogel-loaded targeted nanoparticles (Gel-tNP), and Gel-tNP combined with near-infrared irradiation (NIR, 808 nm), X-ray irradiation (XR, 2 Gy), or both. All treatments were administered at an equivalent 5-FU concentration corresponding to the IC₅₀ determined in panel (A). (E) Intracellular ROS levels in HCT-116 cells measured after 48 h using CellROX™ Deep Red and expressed as fold change relative to Ctrl. Data are mean ± SD ( n = 3). Statistical significance was assessed by one-way ANOVA with multiple-comparisons post-hoc testing. Asterisks indicate comparisons vs. Ctrl (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Brackets indicate the specific intergroup comparisons shown (e.g., dual-modal vs. tri-modal where indicated). “ns” denotes not significant
Airway Infection Model, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/airway+model/pm40902603-245-2-13?v=Charles+River+Laboratories
Average 86 stars, based on 1 article reviews
airway infection model - by Bioz Stars, 2026-07
86/100 stars
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Cytotoxic response and intracellular ROS levels induced by the multimodal hydrogel nanoplatform in CRC and normal colon cell models. (A) Dose–response curve of free 5-FU in HCT-116 cells after 48 h, determined by MTT assay and used to define the experimental IC₅₀. Cell viability was normalized to the Ctrl. For visualization on a logarithmic x-axis, the Ctrl condition is plotted at a nominal concentration of 0.1 µg mL⁻¹. (B–D) MTT-based cell viability after 48 h treatment in (B) HCT-116, (C) SW480, and (D) normal human colon epithelial NCM460 cells. Treatment groups include Ctrl, free 5-FU (5FU), non-targeted nanoparticles (NP), HA-targeted nanoparticles (tNP), blank hydrogel (Gel), hydrogel-loaded targeted nanoparticles (Gel-tNP), and Gel-tNP combined with near-infrared irradiation (NIR, 808 nm), X-ray irradiation (XR, 2 Gy), or both. All treatments were administered at an equivalent 5-FU concentration corresponding to the IC₅₀ determined in panel (A). (E) Intracellular ROS levels in HCT-116 cells measured after 48 h using CellROX™ Deep Red and expressed as fold change relative to Ctrl. Data are mean ± SD ( n = 3). Statistical significance was assessed by one-way ANOVA with multiple-comparisons post-hoc testing. Asterisks indicate comparisons vs. Ctrl (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Brackets indicate the specific intergroup comparisons shown (e.g., dual-modal vs. tri-modal where indicated). “ns” denotes not significant

Journal: Journal of Biological Engineering

Article Title: Hydrogel-mediated tri-modal nanoplatform for localized colorectal cancer therapy via smart chemo–photothermal–radiotherapy

doi: 10.1186/s13036-026-00633-0

Figure Lengend Snippet: Cytotoxic response and intracellular ROS levels induced by the multimodal hydrogel nanoplatform in CRC and normal colon cell models. (A) Dose–response curve of free 5-FU in HCT-116 cells after 48 h, determined by MTT assay and used to define the experimental IC₅₀. Cell viability was normalized to the Ctrl. For visualization on a logarithmic x-axis, the Ctrl condition is plotted at a nominal concentration of 0.1 µg mL⁻¹. (B–D) MTT-based cell viability after 48 h treatment in (B) HCT-116, (C) SW480, and (D) normal human colon epithelial NCM460 cells. Treatment groups include Ctrl, free 5-FU (5FU), non-targeted nanoparticles (NP), HA-targeted nanoparticles (tNP), blank hydrogel (Gel), hydrogel-loaded targeted nanoparticles (Gel-tNP), and Gel-tNP combined with near-infrared irradiation (NIR, 808 nm), X-ray irradiation (XR, 2 Gy), or both. All treatments were administered at an equivalent 5-FU concentration corresponding to the IC₅₀ determined in panel (A). (E) Intracellular ROS levels in HCT-116 cells measured after 48 h using CellROX™ Deep Red and expressed as fold change relative to Ctrl. Data are mean ± SD ( n = 3). Statistical significance was assessed by one-way ANOVA with multiple-comparisons post-hoc testing. Asterisks indicate comparisons vs. Ctrl (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Brackets indicate the specific intergroup comparisons shown (e.g., dual-modal vs. tri-modal where indicated). “ns” denotes not significant

Article Snippet: The cytotoxicity of all treatment groups was evaluated using an MTT assay in two CRC cell lines and one normal human colon epithelial cell model. HCT-116 human colorectal carcinoma cells (ATCC ® CCL-247TM, American Type Culture Collection, USA) and SW480 human colorectal adenocarcinoma cells (ATCC ® CCL-228TM, American Type Culture Collection, USA) were used as CRC models, and normal human colon epithelial cells NCM460 (NCM460DTM; INCELL Corporation LLC, San Antonio, TX, USA) were used to provide an initial in-vitro assessment of relative tolerance in non-malignant colon epithelium.

Techniques: MTT Assay, Concentration Assay, Irradiation